Micrografting of mature stone pine (Pinus pinea L.) trees

Free access article

Issue		Ann. For. Sci. Volume 61, Number 8, December 2004


Page(s)		843 - 845
DOI		10.1051/forest:2004081

Ann. For. Sci. 61 (2004) 843-845
DOI: 10.1051/forest:2004081

Note

Micrografting of mature stone pine (Pinus pinea L.) trees

Millán Cortizo^a, Pablo Alonso^a, Belén Fernández^a, Ana Rodríguez^a, Maria Luz Centeno^b and Ricardo J. Ordás^a

^a Unidad de Fisiología Vegetal, Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/ Catedrático Rodrigo Uría s/n, 33071 Oviedo, Spain
^b Departamento de Biologia Vegetal, Universidad de León, Spain

(Received 1 December 2003; accepted 31 August 2004)

Abstract - This paper describes an in vitro micrografting method for selected mature Pinus pinea L. trees. Needle fascicles of five selected clones were micrografted onto hypocotyls of two weeks-old germinated isolated embryos. Fascicle meristems outgrowth was recorded after one month of culture and the performance of the clones assayed was evaluated. Clones behave statistically different in establishment and development rates. Overall success of our protocol reached 43% of the graftings made.

Résumé - Micro-greffage de clones sélectionnés de Pinus pinea L. Ce rapport décrit une méthode pour le greffage in vitro de Pinus pinea L. Des brachyblastes de cinq clones ont été greffés sur embryons isolés germés, âgés de deux semaines. Le développement des méristèmes fasciculaires a été quantifié pour chaque clone après un mois. Les pourcentages d'établissement et de développement sont statistiquement différents pour les cinq clones. Le taux de succès du greffage réalisé par notre méthodologie s'élève à 43 %.

Key words: micrografting / micropropagation / conifer / in vitro grafting / Pinus pinea

Mots clés : micro-greffage / micro-propagation / conifer / in vitro greffage / Pinus pinea

Corresponding author: Ricardo J. Ordás rordas@uniovi.es

© INRA, EDP Sciences 2005

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