Free access
Ann. For. Sci.
Volume 50, Number Supplement, 1993
Genetics of oaks
Page(s) 344s - 350s
Ann. For. Sci. 50 (1993) 344s-350s
DOI: 10.1051/forest:19930738

Embryogenesis in Quercus petraea

J Jörgensen

Lower Saxony Forest Research Institute, Department of Forest Tree Breeding, 3513 Staufenberg-Escherode, Germany

Abstract - Embryogenesis in Quercus petraea was achieved by culture of zygotic embryos and anthers on modified woody plant medium with benzylaminopurine and 2,4 dichlorophenoxyacetic acid. After counting chromosomes and isoenzyme analysis the embryos from anther culture were subdivided into haploid and somatic clones. Protoplasts that had been isolated from haploid and diploid tissues were regenerated to embryos.

Résumé - Embryogenèse chez Quercus petraea. L'embryogenèse de Quercus petraea a été réalisée par culture d'embryons zygotiques et d'anthères sur du milieu WP contenant de la BAP et du 2,4 D. L'analyse des isoenzymes et le dénombrement des chromosomes ont montré que les embryons issus de culture d'anthères étaient subdivisés en clones haploïdes et somatiques. Les protoplastes isolés à partir de tissus haploïdes et diploides ont été régénérés en embryons.

Key words: somatic embryogenesis / androgenesis / protoplast culture / Quercus petraea

Mots clés : embryogenèse somatique / andfogenése / culture de protoplastes / Quercus petraea

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