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Issue Ann. For. Sci.
Volume 61, Number 8, December 2004
Page(s) 847 - 850
DOI 10.1051/forest:2004083

Ann. For. Sci. 61 (2004) 847-850
DOI: 10.1051/forest:2004083

Note

Salicylic acid up-regulates the expression of chloroplastic Cu, Zn-superoxide dismutase in needles of maritime pine (Pinus pinaster Ait.)

Herlânder Azevedo, Teresa Lino-Neto and Rui M. Tavares

Grupo de Bioquímica e Fisiologia Molecular de Plantas, Centro de Biologia, Departamento de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal

(Received 7 May 2003; accepted 27 April 2004)

Abstract - Several studies have supported a major role of salicylic acid in modulating plant response against abiotic and biotic stresses, by induction antioxidant capacity. In this work, a full-length cDNA encoding a Cu,Zn - superoxide dismutase was isolated by screening a Pinus pinaster needle cDNA library. The predicted protein of 215 amino acid residues has a molecular mass of 22.1 kDa and exhibits a N-terminal transit peptide, which putatively targets the protein to the chloroplast. Treatment of pine seedlings with salicylic acid resulted in the increase of chloroplastic Cu,Zn superoxide dismutase transcript levels in needles, suggesting a role of this isoform in salicylic acid-mediated H2O2 increase in chloroplasts.


Résumé - L'acide salicylique induit une augmentation de l'expression de la Cu,Zn-superoxide dismutase du chloroplaste d'aiguille de pin maritime (Pinus pinaster Ait.). Plusieurs études suggèrent le rôle prédominant de l'acide salicylique dans la modulation de la réponse des plantes aux stress abiotiques et biotiques par induction de la capacité antioxidante. Dans ce travail un ADNc pleine longueur codant pour une Cu,Zn-superoxide dismutase a été isolé par criblage d'une banque d'ADNc d'aiguille de Pinus pinaster. La protéine prédite de 215 acides aminés possède une masse moléculaire de 22,1 kDa et présente, en N-terminal, un peptide signale potentiel d'adressage vers chloroplaste. Le traitement de jeunes plantules de pin par l'acide salicylique induit une augmentation du niveau de transcrit de la Cu,Zn-superoxide dismutase dans les aiguilles, ce qui suggère un rôle de cette isoforme dans l'augmentation des niveaux de H2O2 dans le chloroplaste sous l'effet de l'acide salicylique.


Key words: Pinus pinaster / salicylic acid / chloroplastic Cu,Zn-SOD

Mots clés : Pinus pinaster / acide salicylique / Cu,Zn-SOD chloroplastique

Corresponding author: Rui M. Tavares tavares@bio.uminho.pt

© INRA, EDP Sciences 2005


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