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Ann. For. Sci.
Volume 54, Number 2, 1997
Page(s) 211 - 217
Ann. For. Sci. 54 (1997) 211-217
DOI: 10.1051/forest:19970208

Storage of ectomycorrhizal fungi by freezing

Y Corbery and F Le Tacon

Équipe de microbiologie forestière, Inra, 54280 Champenoux, France

Abstract - Ectomycorrhizal fungi are usually maintained by subculturing at about +25 °C. Ito and Yokohoma (1983) and Jong and Davis (1987) demonstrated that some ectomycorrhizal fungi could be preserved by freezing. We show that the survival of ectomycorrhizal fungi after freezing at -196 °C or -80 °C depends on the cooling rate and on the species or strains. The optimum rate of cooling is -1 °C per min. Thelephora terrestris and Paxillus involutus did not survive any freezing method. The resistance of Cenococcum geophillum to freezing may be related to its tolerance of water stress and of high salinity.

Résumé - La conservation de champignons ectomycorhiziens par congélation. Les champignons ectomycorhiziens sont habituellement conservés par repiquage successif à environ + 25 °C. Les travaux d'Ito et Yokohoma (1983) et ceux de Jong et Davis (1987) ont démontré qu'il était possible de conserver certaines espèces à très basse température. Le présent travail montre que la survie des champignons ectomycorhiziens dépend de la vitesse de congélation à - 196 °C ou - 80 °C ainsi que des espèces ou des souches. La vitesse optimale de congélation est de - 1 °C par minute. Thelephora terrestris et Paxillus involutus ne survivent à aucune méthode de conservation. La résistance de Cenococcum geophillum à la congélation est probablement à mettre en relation avec sa tolérance au stress hydrique et à la salinité.

Key words: freezing / storage / ectomycorrhizal fungi

Mots clés : congélation / conservation / champignons ectomycorhiziens

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