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Table IV

Segregation of alleles at loci where the male. Populus × euramericana (Dode) Guinier parent EA4 is heterozygous.

Code SSR primer locus EA4 (♂) A (♀) 75# (4×) 421 (2×) 422 (2×) 423 (2×)
14 GCPM_2453-1 1 AB CDE ABCDE ADE ADE ADE
41 GCPM_3345-1 2 AB AC ABC AC AC AC
47 GCPM_3559-1 3 AB B AB B AB AB
68 GCPM_432-1 4 AB A AB A A A
105 ORPM_29 5 A0 C0 A0C0 C0 C0 C0
6 B0 DE B0DE BE BE BE

These letters do not necessarily correspond to discrete alleles (e.g. the ``E'' band for GCPM_2453-1 may be the non-specific amplification) and ORPM_29 primer detects two loci, 0 means a null allele.