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Ann. For. Sci.
Volume 59, Number 2, March 2002
Page(s) 155 - 161

Ann. For. Sci. 59 (2002) 155-161
DOI: 10.1051/forest:2002002

Factors involved in Pinus radiata D. Don. micrografting

Mario F. Fragaa, Maria Jesús Cañala, b, Ana Aragonésc and Roberto Rodrígueza, b

a  Lab. Fisiología Vegetal, Dpto. B.O.S., Facultad de Biología Universidad de Oviedo, C/ Catedrático Rodrigo Uría s/n, 33071, Oviedo, Spain
b  Instituto de Biotecnología de Asturias (asociado al CSIC), 33071, Oviedo, Spain
c  Instituto Vasco de Investigación y Desarrollo Agrario (Neiker), Arcaute, s/n, Vitoria, Spain

(Received 1 December 2000; accepted 25 September 2001)

A series of micrografting conditions using needle fascicles from trees of different ages as scions have been evaluated for Pinus radiata D. Don. to increase success of in vitro propagation. Micrografting success depended on the quality of the graft process as well as age, location and development stage of the scion and tree age. 11-month-old scions, taken in January from terminal portions of basal branches show the best micrografting-induced response. Responsiveness of scions decreases with the donor tree age, although this could be overcome by optimising micrografting conditions.

Facteurs impliqués dans le micro-greffage de Pinus radiata D. Don. Différentes conditions de micro-greffage, utilisant comme greffons des brachyblastes provenant d'arbres d'âges différents, ont été comparées afin d'évaluer les possibilités d'améliorer la propagation in vitro de Pinus radiata. Le succès du micro-greffage dépend tout autant de la qualité du processus de greffage que de l'âge, de la localisation et du stade de développement du greffon, ou que de l'âge de l'arbre. Des greffons de 11 mois prélevés en janvier sur la portion terminale de branches de la base de l'arbre donnent les meilleures réponses au micro-greffage. Cette réponse diminue avec l'âge de l'arbre sur lequel ils sont prélevés, bien que ceci puisse en partie être surmonté en optimisant les conditions du micro-greffage.

Key words: reinvigoration / micrografting / maturation / vegetative propagation / Pinus radiata / in vitro culture

Mots clés : vigueur / micro-greffage / maturation / multiplication végétative / Pinus radiata / culture in vitro

Correspondence and reprints: Mario F. Fraga Tel. 985104834; Fax. 985104867;

© INRA, EDP Sciences 2002

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