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Appendix 3

Result of a one-way Ancova with stage of architectural development (ASD = 2) as main factor and relative irradiance (DPFrel, %) as covariables applied to Tachigali melinonii (Harms) Barneby. F -value, level of significance (P) and coefficient of determination (r2) of the model are given. DPFrel, LMA and Jmax a were log transformed prior to analysis.Values of adjusted means (± standard error) were also given (covariate mean = 21%) for the three stages of Architectural development (ASD). Cm and thickness were analyzed with a separate slope model. Vcmax m, Jmax m and Rd m were not linearly correlated with DPFrel and thus they were analyzed with a one way ANOVA. Significant levels: ns, P > 0.05; * P < 0.05; ** P < 0.01 and *** P < 0.001.

ASD DPF rel (%) DPF rel x ASD Model ASD1 ASD2

F F F r2 n = 20 n = 16
H 219*** _ 19.0*** 0.93 45.4± 11 260± 12
D 69.5*** _ 14.8*** 0.82 0.65 ± 0.1 1.66 ± 0.1
Structural traits
LMA 7.46** 104*** _ 0.80 53.2 ± 1.8 62.7 ± 2.0
thickness 10.9** _ 6.19** 0.28 145 ± 5.7 137 ± 6.4
density 12*** 68.7*** _ 0.75 0.3 8± 0.02 0.46 ± 0.02
Nm 26.7*** _ 34.2*** 0.74 23.1 ± 0.8 28.8 ± 0.9
Na 50.9*** 9.37** _ 0.69 1.19 ± 0.05 1.75 ± 0.05
Cm 29.3*** 18.2*** _ 0.65 479 ± 2.3 497 ± 2.6
Chla 23.5*** 46.8*** _ 0.64 552 ± 30 711 ± 34
Chlm 4.14* 145*** _ 0.82 11.8 ± 0.9 12.5 ± 1.0
Physiological traits
Vcmax a 31.2*** 70.3*** _ 0.80 22.9 ± 1.0 32.6 ± 1.1
Jmax a 31.7*** 166*** _ 0.88 36.8 ± 1.6 51.0 ± 1.8
Rd a 1.27ns 14.7*** _ 0.37 1.39 ± 0.2 1.74 ± 0.2
Vcmax m 10.3** ns _ 0.23 430 ± 23 526 ± 18
Jmax m 6.58* 8.08** _ 0.37 686 ± 29 811 ± 33
Rd m 0.92ns ns _ 0.03ns 23.8 ± 2.7 27.8 ± 3.0
Jmax / Vcmax 1.41ns 6.15* _ 0.17ns 1.62 ± 0.05 1.54 ± 0.05
Vcmax/ N 1.13ns 19.6*** _ 0.37 0.27 ± 0.02 0.26 ± 0.02
Jmax/N 3.39ns 53.1*** _ 0.62 0.43 ± 0.02 0.41 ± 0.02
Chl/N 2.77ns 135*** _ 0.82 6.77 ± 0.3 5.83 ± 0.3