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Table I
Properties of the 12 microsatellite markers employed in this study. Genbank accession* numbers are in parentheses under the locus name. The number in parentheses under the allele size range is the number of alleles found for that locus in the Long Hollow black walnut population. The FIS values for the black walnut population is based on a total census of the Long Hollow stand.
| Annealing | Allele size | |||
|---|---|---|---|---|
| Locus | Primer sequences (5′ − 3′) | temp. (°C) | range (bp) | F IS |
| AAG01 | F: GCTTTTGATCAATCGCCCAA | 57 | 145–166 | –0.0951 |
| (AY694137)* | R: ACCCCATTTTGTAGCTTGGA | (8) | ||
| WGA06 | F: CCATGAAACTTCATGCGTTG | 57 | 134–172 | 0.0243 |
| (AY333949) | R: CATCCCAAGCGAAGGTTG | (18) | ||
| WGA24 | F: TCCCCCTGAAATCTTCTCCT | 55 | 222–248 | 0.0119 |
| (AY333950) | R: TTCTCGTGGTGCTTGTTGAG | (14) | ||
| WGA27 | F: AACCCTACAACGCCTTGATG | 55 | 199–245 | 0.0112 |
| (AY333951) | R: TGCTCAGGCTCCACTTCC | (19) | ||
| WGA32 | F: CTCGGTAAGCCACACCAATT | 58 | 163–217 | 0.0208 |
| (AY333952) | R: ACGGGCAGTGTATGCATGTA | (28) | ||
| WGA69 | F: TTAGTTAGCAAACCCACCCG | 50 | 164–188 | 0.0483 |
| (AY333953) | R: AGATGCACAGACCAACCCTC | (10) | ||
| WGA72 | F: AAACCACCTAAAACCCTGCA | 55 | 135–159 | 0.1272 |
| (AY333954) | R: ACCCATCCATGATCTTCCAA | (11) | ||
| WGA76 | F: AGGGCACTCCCTTATGAGGT | 58 | 148–166 | –0.0019 |
| (AY688266) | R: CAGTCTCATTCCCTTTTTCC | (10) | ||
| WGA82 | F: TGCCGACACTCCTCACTTC | 57 | 140–234 | –0.0003 |
| (AY333956) | R: CGTGATGTACGACGGCTG | (38) | ||
| WGA89 | F: ACCCATCTTTCACGTGTGTG | 57 | 179–233 | 0.0402 |
| (AY352440) | R: TGCCTAATTAGCAATTTCCA | (25) | ||
| WGA90 | F: CTTGTAATCGCCCTCTGCTC | 55 | 142–178 | 0.0379 |
| (AY352441) | R: TACCTGCAACCCGTTACACA | (19) | ||
| WGA97 | F: GGAGAGGAAAGGAATCCAAA | 57 | 149–189 | 0.0242 |
| (AY352442) | R: TTGAACAAAAGGCCGTTTTC | (17) |