Ann. For. Sci.
Volume 67, Number 8, December 2010
|Number of page(s)||8|
|Published online||28 October 2010|
Pollination dynamics in a Douglas-fir seed orchard as revealed by pedigree reconstruction
Department of Forest Sciences, Faculty of Forestry, The University
of British Columbia, 2424 Main
British Columbia, V6T 1Z4
2 Department of Dendrology and Forest Tree Breeding, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 21 Praha 6, Czech Republic
3 Saanich Forestry Centre, Western Forest Products Inc, 8067 East Saanich Road, Saanichton, British Columbia, V8M 1K1 Canada
4 British Columbia Ministry of Forests and Range, Research Branch, Victoria, British Columbia, V8W 9C2 Canada
5 SelectSeed Company Limited, 3370 Drinkwater Road, Duncan, British Columbia, V9L 5Z2 Canada
* Corresponding author: firstname.lastname@example.org
Accepted: 31 March 2010
• Pollination dynamics was studied in a Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seed orchard using 8 nuclear microsatellite markers and pedigree reconstruction.
• The seed orchard consisted of 49 parents (clones). Cone-crop management included bloom delay and supplemental mass pollination (SMP) using 12 internal and 4 external pollen donors.
• A random sample of 801 bulk seeds was genotyped for both haploid megagametophyte and corresponding diploid embryo.
• Using the parental population’s multilocus genotypes, full pedigree reconstruction generated all the information needed to estimate the maternal, paternal, and parental reproductive success, selfing, pollen contamination, and pollination success of the 4 external pollen donors.
• Maternal, paternal, and parental reproductive success varied with 80% of gametes being produced by 23, 45, and 37% of the orchard’s parents, respectively, resulting in a drastically reduced effective population size as compared to the census number (14 vs. 53).
• Selfing, pollen contamination, and aggregate SMP success (internal and external) were estimated to be 15.2, 10.4, and 15.0%, respectively.
• Full pedigree reconstruction was effective in unraveling the orchard’s pollination dynamics and both female and male reproductive success.
Key words: seed orchard / DNA fingerprinting / pedigree reconstruction / mating dynamics / Pseudotsuga menziesii
© INRA, EDP Sciences, 2010