| Issue |
Ann. For. Sci.
Volume 67, Number 8, December 2010
|
|
|---|---|---|
| Article Number | 808 | |
| Number of page(s) | 8 | |
| Section | Original articles | |
| DOI | https://doi.org/10.1051/forest/2010044 | |
| Published online | 28 October 2010 | |
Original article
Pollination dynamics in a Douglas-fir seed orchard as revealed by pedigree reconstruction
1
Department of Forest Sciences, Faculty of Forestry, The University
of British Columbia, 2424 Main
Mall, Vancouver,
British Columbia, V6T 1Z4
Canada
2
Department of Dendrology and Forest Tree Breeding, Faculty of
Forestry and Wood Sciences, Czech University of Life Sciences Prague,
Kamýcká 129, 165 21
Praha 6, Czech
Republic
3
Saanich Forestry Centre, Western Forest Products
Inc, 8067
East Saanich Road, Saanichton, British Columbia, V8M
1K1
Canada
4
British Columbia Ministry of Forests and Range, Research
Branch, Victoria,
British Columbia, V8W 9C2
Canada
5
SelectSeed Company Limited, 3370 Drinkwater Road, Duncan, British Columbia, V9L
5Z2
Canada
* Corresponding author: y.el-kassaby@ubc.ca
Received:
18
November
2009
Accepted:
31
March
2010
• Pollination dynamics was studied in a Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) seed orchard using 8 nuclear microsatellite markers and pedigree reconstruction.
• The seed orchard consisted of 49 parents (clones). Cone-crop management included bloom delay and supplemental mass pollination (SMP) using 12 internal and 4 external pollen donors.
• A random sample of 801 bulk seeds was genotyped for both haploid megagametophyte and corresponding diploid embryo.
• Using the parental population’s multilocus genotypes, full pedigree reconstruction generated all the information needed to estimate the maternal, paternal, and parental reproductive success, selfing, pollen contamination, and pollination success of the 4 external pollen donors.
• Maternal, paternal, and parental reproductive success varied with 80% of gametes being produced by 23, 45, and 37% of the orchard’s parents, respectively, resulting in a drastically reduced effective population size as compared to the census number (14 vs. 53).
• Selfing, pollen contamination, and aggregate SMP success (internal and external) were estimated to be 15.2, 10.4, and 15.0%, respectively.
• Full pedigree reconstruction was effective in unraveling the orchard’s pollination dynamics and both female and male reproductive success.
Key words: seed orchard / DNA fingerprinting / pedigree reconstruction / mating dynamics / Pseudotsuga menziesii
© INRA, EDP Sciences, 2010