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Genotypic variation and water deficit effect on traits related to DNA methylation in six Populus deltoides × P. nigra hybrids growing in glasshouse in well-watered (white bar) or in water deficit (black bar) conditions. Studied traits encompassed: (A) percentages of methylation of cytosine residues (%mC) and (B) total DNMT activity (see Materials and Methods section for details). For each graph, G indicates the genotype effect, T the treatment effect and (GxT) genotype by treatment effect. Means are accompanied by their standard errors SE (n = 6). Significant differences between well-watered and water deficit conditions are indicated by asterisk: * P ≤ 0.05, ** P ≤ 0.01 and *** P ≤ 0.001 (C) immunodetection in the six genotypes of isoforms of DNMT class I. C (Control) represents plants in well-watered condition and S (Stressed) plants in water deficit condition. Sizes of the different isoforms are indicated in kiloDalton.