Table I
Prunus avium L. Germination after seed storage with water content (w.c.) of 7.7% before dormancy release (A) and w.c. of 7.8% after dormancy release (B) for 1 or 2 y at − 3 °C (control) or − 196 °C. Tukey test, the values marked with the same letter are not significantly different at P < 0.05, provenance Zwierzyniec (Roztocze, SE Poland).
| Storage time | Storage | Germination (%) | ||
|---|---|---|---|---|
| temperature | storage → stratification | stratification → storage | ||
| °C | (A) | (B) | ||
| 1 y | –3 | 29 ab | 8 cd | |
| –196 | 37 a | 5 d | ||
| 2 y | –3 | 45 a | 6 d | |
| –196 | 27 abc | 13 bcd | ||
| ANOVA | df | df ERROR | F | P |
| S. Time | 1 | 24 | 1.31 | 0.2632 |
| S. Method | 1 | 24 | 70.77 | 0.0000* |
| S. Temp. | 1 | 24 | 0.21 | 0.6529 |
| S. Time • S. Method | 1 | 24 | 0.16 | 0.6938 |
| S. Time • S. Temp. | 1 | 24 | 0.25 | 0.6248 |
| S. Method • S. Temp. | 1 | 24 | 0.65 | 0.4279 |
| S.TimeS • S. Method • S. Temp. | 1 | 24 | 7.72 | 0.0104 |
ANOVA – variables: S. Time – time of seed storage (1 or 2 y), S. Method – the method of seed storage (stored before stratification – A or after stratification – B), S. Temp. – the storage temperature (− 3 °C or − 196 °C).
Nongerminated seeds: decayed (52–92%), healthy seed (2–5%) and up to 1% empty.Germination from nonfrozen seeds after stratification (without seed desiccation): 30% (nongerminated seeds: 66% decayed, 4% healthy).Germination from nonfrozen seeds after their stratification and desiccation (to w.c. of 7.8%): 10% (nongerminated seeds: 89% decayed, 1% healthy).